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The main objective of this study was to discover MYB domain genes in cocoa, which are known to be involved in the biosynthesis of secondary metabolites, to improve our understanding of their function in relation to the regulation of polyphenol biosynthesis in cocoa. As a result, 16 full-length MYB genes belonging to subgroup 2 were isolated from two Arabidopsis and one tobacco cultivars, and their phylogenetic relationships were found. Subsequently, the tissue-specific expression of MYB domain genes in cocoa was investigated, resulting in the identification of six MYB genes that are expressed in roots and/or leaves. Functional analyses revealed that one MYB gene from subgroup 2C, MYB1 from subgroup 2F, and MYB2 from subgroup 2H play roles in the regulation of anthocyanin and proanthocyanidin biosynthesis in Arabidopsis seedlings. The overexpression of the entire MYB genes induced the accumulation of anthocyanin and catechin in Arabidopsis seedlings. Further, two MYB genes from subgroup 2A were transiently overexpressed in Arabidopsis leaves. These results suggest that MYB domain genes from subgroup 2 may be involved in the regulation of polyphenol production in cocoa and might also be useful in the improvement of polyphenol synthesis in plants.COVID19 Update
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