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The formation of dental plaque and caries was investigated in relation to the life cycle of Streptococcus mutans. Sterile oral cells were coated on glass slides, and the adherence, growth, and metabolic activities of these cells, expressed as the number of cells per slide, were measured. In the course of colonization by S. mutans, the accumulation of a bacterial cell number peaked at day 2, and the accumulation of a carbohydrate-binding protein (CBP)-positive cell number peaked on day 4 and then gradually decreased. However, the total bacterial cell number was constant during the first 5 days of colonization. Two-dimensional electrophoresis showed that the CBP activity, which increased after 4 days of colonization, was glycolytic phosphofructokinase. In the absence of dietary sucrose, the formation of dental plaque was slightly slower than that in the presence of sucrose. Sucrose addition accelerated the formation of dental plaque during the 5-day life cycle of S. mutans. In a longitudinal study in which planktonic bacteria were aspirated from the saliva of the same subjects, only active bacteria were detected, irrespective of the diet. The metabolic activity of the active bacteria peaked at the early stage of colonization, suggesting that the ability of these bacteria to adhere to and colonize surfaces is related to the concentration of synthesized carbohydrates.SPONTANEANATION with Paul F. Tompkins #192

Paul F. Tompkins welcomes everyone to the Audience Appreciation Hour, and gets an earful about everything from Carrie’s sex tape to the Big Mouth Awards, starting with what have been the best episodes of Samantha Bee’s new late night show. Then, Craig Robinson and Danielle Schneider visit to talk about the new season of The Office, and get drunk off Scotch. Later, Ben Schwartz joins Paul and John Hodgman to name more of the stars from the worst movies of all time. And as always, Eban (only the best) Schletterstein will judge the Best Performance by an Integer at the 55th Annual Grammy
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